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Daniele De Rossi is set to miss Italy''s quarter-final clash with Germany in Bordeaux after sitting out the Azzurri's early morning training session on Saturday.
The Roma midfielder suffered a thigh injury during the Italian's 2-0 Round of 16 win over Spain, and, although he returned to full training on Friday afternoon, the 32-year-old worked away from the main squad on Saturday morning.
Juventus midfielder Stefano Sturaro is expected to start against the world champions in his place, as Thiago Motta is suspended and Antonio Candreva injured.
Italy boss Antonio Conte had hinted on Friday that De Rossi was not fit enough to start against Germany, despite his "decent" physical condition,
"We won’t get ahead of ourselves, as these are games to be played at 120 per cent and very, very physically demanding," he said.
"We must be not in good condition, but great. I have a lot of faith in the entire group."
Germany coach Joachim Loew, who describes De Rossi as Conte's "interpreter on the pitch" says the Italian coach has quality players throughout his 23-man squad.
"I don’t know if De Rossi will start tomorrow, as Italy are very flexible on their starting XI and you must always expect surprises from them," he said.
“The principle objective is to win the tournament and everyone has quality. I believe whoever wins tomorrow is the favourite to lift the trophy.”