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Ar Mad and Simonsig will both miss next month's Cheltenham Festival through injury.
Arkle Trophy contender Ar Mad won at Plumpton on Monday, his final run before the National Hunt Festival, but he was found to be lame shortly after the race.
On Tuesday trainer Gary Moore revealed the six-year-old had suffered a condylar fracture, which will see him miss the remainder of the season.
"It is a front leg. He didn't look like a lame horse going round there [Plumpton]," Moore said.
"That's the end of his season. The prognosis is good. He will be back all being well next season. He has had surgery to insert a screw in the leg."
News of Ar Mad's injury comes less than a day after stablemate Violet Dancer was ruled out for the rest of the campaign with the same problem.
Meanwhile, Nicky Henderson has confirmed that Simonsig will not be heading to Prestbury Park.
Henderson had hoped to run the ten-year-old in either the Queen Mother Champion Chase or the Ryanair Chase, but the Seven Barrows handler has run out of time to get him ready for Cheltenham.
"We took him out of Cheltenham this morning as we're obviously not going to get there. It's just a splint problem that has been annoying us for ages," Henderson confirmed.
"We have been dragging it out and we're not going to make it. We'll look now at Aintree, Punchestown or wherever. We'll keep trying, we're not going to give up with him."