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Postponed has been ruled out of Saturday's King George VI And Queen Elizabeth Stakes due to a respiratory infection.
Roger Varian's charge has looked in superb form this season, winning the Dubai Sheema Classic and Coronation Cup and he was the warm favourite to add to his victories in Saturday's showpiece at Ascot.
On Wednesday trainer Roger Varian confirmed the five-year-old had returned an unsatisfactory scope just days before the race.
"Up until this point, Postponed had not given us any worry during his training regime," the trainer wrote on his website. "However, we felt Postponed did not work with his usual zest this morning and a subsequent scope showed he is suffering from a respiratory infection.
"This news is obviously very disappointing and it is a real shame for Sheikh Mohammed Obaid, the team and the racing public that he will not line up on Saturday.
"But, as is always the case, the interests of the horse must come first. If he is not 100 per cent, the only sensible thing to do is not to run."
The trainer went on: "We will let the horse recover from this setback before making a plan of where he runs next.
"We still have many big days ahead to look forward to."