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Cheltenham Gold Cup winner Don Cossack, Cue Card and Vautour will all feature in the Punchestown Gold Cup on 27 April.
Nine of the 11 Grade 1 winners from Aintree's Grand National meeting, and nine of the 13 Grade 1 winners from Cheltenham will race over jumps at the racecourse near Naas.
Cue Card and Don Cossack are among 20 entries that also include the Cheltenham second, third and fourth - Djakadam, Don Poli and Carlingford Lough.
"The plan always was to go on to Punchestown and I don’t see any reason to change that," trainer Colin Tizzard said after Cue Card's Betfred Bowl win at Aintree.
"Hopefully [Cue Card and Don Cossack] jump a clear round. Don Cossack’s fallen once, we’ve fallen once and hopefully they both jump clear and we’ll see who finishes in front."
Trainer Gordon Elliott watched Don Cossack ride over a mile and a half at Punchestown on Monday, and confirmed that the nine-year-old will race in Punchestown at the end of the month.
He said: “We haven’t done anything serious with him since then and today was the first time that he’s really stretched his legs since Cheltenham.
“Cue Card is a horse I’ve got massive respect for. It’s going to be a very good race at Punchestown. He [Don Cossack] will have one more good bit of work and then it’s all systems go.”
Ryanair Chase winner Vautour's trainer Willie Mullins said on Monday: "There’s a very good chance Vautour will run in the Gold Cup. He’s as fit as a flea, and as long as he’s sparkling at home he’ll take his chance."