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Jamaica claimed glory in the 4x100m final at Rio 2016, giving Usain Bolt his ninth Olympic gold medal.
Bolt won the 100m and 200m individual titles, along with relay success, at both Beijing 2008 and London 2012.
Having already secured 100m and 200m in Brazil, he combined with Asafa Powell, Yohan Blake and Nickel Ashmeade to take gold in the relay final with a time of 37.27 seconds.
Japan finished in a surprise second place, while an illegal baton change saw the United States disqualified, allowing Canada to claim bronze.
"There you go - I'm the greatest," said Bolt, who intends to retire after the 2017 World Championships, following Jamaica's 4x100m triumph in Rio.
USA sprinter and long jumper Carl Lewis, Bolt and Finnish long-distance legend Paavo Nurmi are the only other athletes to have won nine Olympic gold medals.
"It's great to be in the history books as one of the greatest," added Bolt. "I'm proud of myself.
"I never knew this would happen when I started out. My team came through for me tonight. As long as we got the baton round, it was never in doubt."
Great Britain were classified in fifth place in the relay final, following USA's disqualification.