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So Mi Dar will miss the Investec Oaks after being found to be lame.
The daughter of Da Re Mi was installed as the second favourite for the Classic after she beat Fireglow to land the Musidora Stakes at York's Dante Festival last week.
Trainer John Gosden had been of the opinion that his filly would provide a real threat for 1000 Guineas winner Minding in the June 3 feature, but she will not be heading to Epsom after being found to be lame.
"It was one of those mornings, you get. I was perfectly happy [with her] this morning. We walked them for 10-15 minutes, nice little trot, not so much to warm them up, but just to see how they are, and she was lame on her left-hind," Gosden said.
"It's unfortunate for Andrew and Madeleine (Lloyd Webber, owners). Madeleine being a real horse woman, she's ridden a lot, she knows. I phoned them straight away.
"I looked at it, she is not right. We would call it a 1-2 out of 5 if you graded it on lameness. It's the old story. She may well come right in 10 days' time. But I'm afraid the horse comes very much first. You can't send a horse to the Oaks who has just been walking for 10 days. We will do what is right for the filly and I'm afraid she is a non-runner."
With Epsom off the agenda, Gosden hopes to get her ready for the Irish Oaks.
"It's a disappointment, a real disappointment," the trainer added, "She's a gorgeous filly. She won in great style and we were all thrilled but so often with these things if a little something started in the race you don't often see it straight away.
"She was perfectly good. But this morning just as she trotted off I watched and she was favouring her near-hind. She may well come right, but it's not a little bruise in the foot, it's a little higher up, and we're just going to take her time to get her right.
"Our role as a trainer is to do what is right by the horse. As soon as I saw that, I got straight on the phone, and it's wonderful that it is people who understand.
"I'm sure she'll probably be back for the Irish Oaks, but sadly not for Epsom.