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Un De Sceaux and Sire De Grugy are set to renew their rivalry this weekend in the Sodexho Clarence House Chase at Ascot on Saturday.
The pair are among just five declared runners for the Grade One challenge which will provide a good indication of how they are progressing ahead of the Cheltenham festival in March.
The Willie Mullins-trained Un De Sceaux remains the favourite for the Queen Mother Champion Chase at Prestbury Park despite falling two fences from home when travelling well in front in the Paddy Power Chase at Leopardstown last month.
However, he will be put to the test this weekend against his old rival Sire who won this race on his way to taking the Champion Chase crown two years ago.
The Gary Moore-trained horse has been in decent form this winter, winning the Tingle Creek at Sandown for a second time and just losing out to Sprinter Sacre in the Desert Orchid Chase at Kempton last month.
Moore is also running Traffic Fluide in the race, while Mullins has withdrawn his only other entry, Felix Yonger.
Nicky Richards saddles Simply Ned, who was runner-up in the Paddy Power Chase at Leopardstown, with Paul Nicholls' Vibrato Valtat, who was three and three-quarter lengths behind Sire De Grugy when third at Kempton last time out, completing the quintet.